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Development of surface-enhanced Raman scattering-based assays with bioorthogonal Raman reporters for quantification of nucleic acids
http://hdl.handle.net/10212/2625
http://hdl.handle.net/10212/26252d8a443b-c279-4e23-a5a8-2dc36552436c
| 名前 / ファイル | ライセンス | アクション |
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全文 (3.1 MB)
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内容・審査結果の要旨 (139.4 KB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2024-08-08 | |||||||||
| タイトル | ||||||||||
| タイトル | Development of surface-enhanced Raman scattering-based assays with bioorthogonal Raman reporters for quantification of nucleic acids | |||||||||
| 言語 | en | |||||||||
| その他のタイトル | ||||||||||
| その他のタイトル | 生体直交型ラマンレポーターを利用した表面増強ラマン散乱測定法の開発と核酸検出法への応用 | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
太田, 良
× 太田, 良
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Surface-enhanced Raman scattering | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Nucleic acids detection | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Gold nanoparticles | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Bioorthogonal Raman reporters. | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Liquid biopsy has attracted much attention as the non-invasive diagnostic technique for early diagnosis of cancer, viral diseases, and infectious diseases. Notably, nucleic acid-based diagnostic systems have good potential to sensitively, faithfully, and rapidly diagnose in comparison with the conventional biopsy. Quantitative polymerase chain reaction (qPCR) is widely used for highly-sensitive quantification of the nucleic acids in laboratories. However, since qPCR has the complicated and time-consuming procedure for enzymatic amplification of nucleic acids, qPCR has been poorly utilized as diagnostic methods. Therefore, the development of alternative diagnostic technique for quantifying nucleic acids without nucleic acid amplification is desired. Recently, the concern over the approach for detection of nucleic acids using surface-enhanced Raman scattering (SERS) has risen in biomedical fields. SERS amplifies Raman signal of the molecule adsorbed on the metal nanoparticle surfaces by 108- to 1010-fold, allowing for detection at the single molecule level. However, SERS-based assays for accurately quantifying target molecules present challenges because of the poor reproducibility of SERS signals. In this thesis, I developed novel SERS-based assays for accurately detecting nucleic acids using biorthogonal Raman reporter-modified SERS probes, which have a Raman signal in the biologically silent region (1800?2800 cm-1). Chapter 1 concerns the development of a novel sandwich-type assay for simple and facile detection of nucleic acids using SERS probes composed on a gold nanorod as a SERS substrate and 4-cyano-N-(2-mercaptoethyl)benzamide (4CMB), which has a Raman signal at 2240 cm-1. 500 pM of target DNAs were detected by the sandwich-type assay in a sequence-selective manner. Chapter 2 concerns the development of a novel ratiometric SERS assay for reliable quantification of nucleic acids using SERS probe 1 with 3-mercapto-N-[4-(2-phenylethynyl)phenyl]propenamide (PEP) as reporter molecules and SERS probe 2-coated silica beads with N-(4-ethynylphenyl)-3-mercaptopropanamide (EP) as internal standard, respectively, to improve the reproducibility of SERS intensities. Target DNAs were detected by the ratiometric assays down to 86 pM. Normalization of SERS intensities by internal standard leads to suppression of the variation of SERS signals, allowing for quantification and automatic detection of target nucleic acids. | |||||||||
| 言語 | en | |||||||||
| 日付 | ||||||||||
| 日付 | 2021-03-25 | |||||||||
| 日付タイプ | Issued | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第985号 | |||||||||
| 学位名 | ||||||||||
| 言語 | ja | |||||||||
| 学位名 | 博士（学術） | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2021-03-25 | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 14303 | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関名 | 京都工芸繊維大学 | |||||||||
