WEKO3
アイテム
Studies on Drosophila genes related to cancer and their roles in cell metabolism
http://hdl.handle.net/10212/2254
http://hdl.handle.net/10212/2254132b5eac-0c35-4897-8313-3f2a7c340a57
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全文 (1.6 MB)
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内容・審査結果の要旨 (318.6 KB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2017-07-03 | |||||||||
| タイトル | ||||||||||
| タイトル | Studies on Drosophila genes related to cancer and their roles in cell metabolism | |||||||||
| 言語 | en | |||||||||
| その他のタイトル | ||||||||||
| その他のタイトル | ショウジョウバエの癌関連遺伝子とその細胞代謝における役割についての研究 | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
ニィエン タイン, ビン
× ニィエン タイン, ビン
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | SCOX | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | mitochondria | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | ATP | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | ROS | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | SCO2 | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | lifespan | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Cabeza | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | retina | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | EGFR pathway | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Drosophila | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Amyotrophic lateral sclerosis | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Mitochondria are cellular occupants which are referred as the power plant of the eukaryotes cells. They play important roles in producing adenosine triphosphate through the oxidative phosphorylation, maintaining the environment within our cells by control cytolysis calcium concentration. Mitochondrial dysfunction is reported in aging and many severe diseases such as neurodegenerative diseases, cancer. Although mitochondrial dysfunction is the energy production problem, the mechanisms of mitochondrial dysfunction in all cases are yet unclear. In my study, I focus on two of genes: SCO2 (synthesis of cytochrome c oxidase 2) which is associated to COX deficiency; and FUS (Fuses in Sarcoma) which is related to neurodegenerative diseases. In the first part, I investigate the in vivo role of SCOX (homologue of human SCO2 in Drosophila melanogaster) by generating the SCOX-knockdown flies and full-length SCOX transgenic flies. The results are that knockdown of SCOX in all cells to be associated with lethality in larvae or pupil while the full length SCOX transgenic flies showed a longer lifespan more than, correlated with the decrease and increase in ATP level, respectively. Finally, when cultured on paraquat-added medium, full length SCOX transgenic flies exhibited an elongated lifepan. I hypothesized that SCOX play an important role in ATP consumption and production. In the next part, I study on Caz-knockdown flies to investigate the in vivo roles of Cabeza (Caz, which is a FUS homologue in Drosophila melanogaster). The results indicated that Caz-knockdown induces apoptosis and inhibits differentiation of cone cells. Moreover, mutation of EGFR pathway-related genes suppressed the rough eye phenotype induced by Caz-knockdown and the rhomboid-1 mutation rescued the fushion of cone cells and ommatidia observed in Caz-knockdown flies. That suggests Caz negatively regulates the EGFR singalling pathway in Drosophila melanogaster. More in vivo and in vitro experiments will be done to clarify other genetic functions of SCOX and Caz, and the mechanism of mitochondrial dysfunction and neurodegenerative disorders can be clearly elucidated. | |||||||||
| 言語 | en | |||||||||
| 日付 | ||||||||||
| 日付 | 2015-03-25 | |||||||||
| 日付タイプ | Issued | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第734号 | |||||||||
| 学位名 | ||||||||||
| 言語 | ja | |||||||||
| 学位名 | 博士（学術） | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2015-03-25 | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 14303 | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関名 | 京都工芸繊維大学 | |||||||||
