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Study on yeast response to vanillin, a fermentation inhibitor derived from lignocellulosic biomass
http://hdl.handle.net/10212/2343
http://hdl.handle.net/10212/2343ccac63f3-7991-427b-bacb-323a13a63639
| 名前 / ファイル | ライセンス | アクション |
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内容の要約 (69.9 KB)
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内容・審査結果の要旨 (332.5 KB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2018-06-04 | |||||||||
| タイトル | ||||||||||
| タイトル | Study on yeast response to vanillin, a fermentation inhibitor derived from lignocellulosic biomass | |||||||||
| 言語 | en | |||||||||
| その他のタイトル | ||||||||||
| その他のタイトル | リグノセルロース系バイオマス由来発酵阻害物質バニリンに対する酵母のストレス応答の解析 | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
グェン チ ミジェン
× グェン チ ミジェン
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Saccharomyces cerevisiae | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | lignocellulosic biomass | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | vanillin | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | oxidative stress | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | G6PDH | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | pentose phosphate pathway | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | translational repression | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | ADH6 | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | ADH7 | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | VIE1 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Vanillin is a phenolic aldehyde compound generated during the saccharification pretreatment of the lignocellulosic biomass in bioethanol production. It has been reported that vanillin inhibits yeast growth, represses translation and induces the formation of ribonucleoprotein granules such as P-bodies and stress granules in Saccharomyces cerevisisae. I herein demonstrated that vanillin causes oxidative stress and induces mitochondrial fragmentation in yeast cells, providing some new information regarding the effects of vanillin on yeast. Additionally, I found that the null mutant of glucose-6-phosphate dehydrogenase (G6PDH), which catalyzes the rate-limiting NADPH-producing step in the pentose phosphate pathway (PPP), was more susceptible to vanillin than the wild-type, suggesting the importance of G6PDH and PPP in vanillin tolerance. It has been known that vanillin can be reduced to its less toxic form, vanillyl alcohol, by the yeast NADPH-dependent medium chain alcohol dehydrogenases, Adh6 and Adh7 in vitro. In this study, I verified the roles of these proteins in vanillin tolerance in vivo. I also showed that although both the ADH6 and ADH7 genes were transcriptionally upregulated, the protein levels of Adh7 but not Adh6 were significantly increased in the presence of high concentration of vanillin, indicating that the ADH7 mRNA was preferentially translated under severe vanillin stress. In addition to the ADH7 gene, we found that the expression of VIE1 gene, coding an unknown protein, was also significantly induced upon vanillin treatment. Both the ADH7 and VIE1 promoter can drive the expression of non-native genes under severe vanillin stress despite the overall translation repression, suggesting that these promoters are useful to overexpress genes in bioethanol production. Importantly, the VIE1 promoter also enabled the increased expression of genes in the presence of furfural and 2-hydroxymethylfurfural, other fermentation inhibitors derived from lignocellulosic biomass. Therefore, the VIE1 promoter would be more practical in modification of gene expression in order to improve the efficiency of ethanol production from lignocellulosic biomass. | |||||||||
| 言語 | en | |||||||||
| 日付 | ||||||||||
| 日付 | 2016-09-26 | |||||||||
| 日付タイプ | Issued | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第807号 | |||||||||
| 学位名 | ||||||||||
| 言語 | ja | |||||||||
| 学位名 | 博士（学術） | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2016-09-26 | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 14303 | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関名 | 京都工芸繊維大学 | |||||||||
