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Biological analyses of transcriptional regulators in Drosophila
http://hdl.handle.net/10212/2520
http://hdl.handle.net/10212/252017ce6825-ca37-477c-9a6b-5e8bdb86b66d
| 名前 / ファイル | ライセンス | アクション |
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内容の要約 (15.6 KB)
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内容・審査結果の要旨 (192.6 KB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2022-04-16 | |||||||||
| タイトル | ||||||||||
| タイトル | Biological analyses of transcriptional regulators in Drosophila | |||||||||
| 言語 | en | |||||||||
| その他のタイトル | ||||||||||
| その他のタイトル | キイロショウジョウバエにおける転写制御因子の生物学的解析 | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
田中, 領
× 田中, 領
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Drosophila | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | LMO7 | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Smallish | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Eye development | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | G9a | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Epigenetics | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Starvation stress | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Gene expression is accurately and dynamically modulated by various mechanisms including epigenetic regulation and transcription DNA to RNA. These regulations are fundamental mechanism for development, tissue homeostasis and behavior. 1. Functional analysis of Smallish during Drosophila development Smallish (Smash), the Drosophila homologue of human LMO7, was reported as a key regulator of Drosophila embryogenesis associated with actomyosin contractility. Although smash mRNA is expressed throughout Drosophila development, only Smash function at the adherence junction in embryonic epithelial cells has been revealed. Here, I demonstrated that the eye imaginal disc-specific smash knockdown induced eye morphological aberrations associated with increased apoptosis. Moreover, immunohistochemical analyses revealed that Smash mainly localized to the nucleus in several tissues, including eye imaginal discs. As is the case with LMO7, the smash knockdown in eye imaginal discs decreased the expression of the ote and bocks genes known as the Drosophila homologue of the emerin gene. Furthermore, down-regulated ote and bocks expression induced apoptosis. Taken together, these results indicate that Smash functions in proper Drosophila eye development mediated by the regulation of ote and bocks gene expression.2. G9a is a key regulator of the starvation-induced behaviors in Drosophila melanogaster Organisms are exposed to starvation stress in nature. In spite of the importance of behavioral strategies under the starvation, the underlying mechanisms have not been fully understood. Here, I revealed that Drosophila G9a (dG9a), one of histone methyltransferases, plays a key role for the starvation-induced behaviors. RNA-seq and RT-qPCR analysis revealed that the expression of gustatory receptor genes for sensing sugar are increased in dG9a null mutant under the starvation condition. Consistent with its up-regulation, proboscis extension reflex tests indicated that the sucrose sensitivity under starvation conditions was significantly increased. Moreover, the locomotion activity was promoted in starved dG9a null mutant. This hyperactivity is caused by down-regulation of insulin-like peptide genes expression under the dG9a depletion, which genes are required for the suppression of starvation-induced hyperactivity. Moreover, refeeding of wild type flies after starvation conditions restores these phenotypes. These data suggest that dG9a functions as a key regulator for the decision of behavioral strategies under starvation conditions. | |||||||||
| 言語 | en | |||||||||
| 日付 | ||||||||||
| 日付 | 2019-03-25 | |||||||||
| 日付タイプ | Issued | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第910号 | |||||||||
| 学位名 | ||||||||||
| 言語 | ja | |||||||||
| 学位名 | 博士（学術） | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2019-03-25 | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 14303 | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関名 | 京都工芸繊維大学 | |||||||||
