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Function of lipid storage droplet-1 and 2 in development of Drosophila melanogaster
http://hdl.handle.net/10212/2543
http://hdl.handle.net/10212/2543df859cf8-f1c9-4414-a354-3d4ccf7db060
| 名前 / ファイル | ライセンス | アクション |
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内容の要約 (42.2 KB)
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内容・審査結果の要旨 (178.5 KB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2022-04-20 | |||||||||
| タイトル | ||||||||||
| タイトル | Function of lipid storage droplet-1 and 2 in development of Drosophila melanogaster | |||||||||
| 言語 | en | |||||||||
| その他のタイトル | ||||||||||
| その他のタイトル | ショウジョウバエlipid storage droplet-1および 2遺伝子の発生過程における機能 | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
チャン ドゥイ ビン
× チャン ドゥイ ビン
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Drosophila | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Lipid storage droplet-1 | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Lipid storage droplet-2 | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Development | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Development | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | dFoxO | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | JNK signaling pathway | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | LSD-1 may positively control JNK signaling pathway | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | which is required for thorax development | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Role of LSD-2 in the development of Drosophila wings via genetically interaction with dFoxO | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Lipid droplets comprise neutral lipids that are enclosed by a monolayer of phospholipids. The surface of a lipid droplet is decorated with several types of proteins such as structural proteins, lipid-synthesis enzymes, lipases, and membrane-trafficking proteins. Notably, Drosophila and mammals share these lipid metabolism-related genes and basic metabolism function. Perilipins are known to be involved in regulating the accessibility of lipases; e.g., mammalian adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL), by either recruiting these enzymes or preventing their access to lipid droplets. Perilipins regulate ATGL activation through the interaction with comparative gene identification 58 (CGI-58), which is an ATGL coactivator. Also, the role of perilipin 1 locus was identified as obesity risk factors, and perilipin 2 participates in the pathogenesis of diet-induced insulin resistance. Drosophila encodes two perilipin family member, Lsd-1/perilipin-1 and Lsd-2/perilipin-2. LSD-1 indiscriminately labels lipid droplets of varying size whereas LSD-2 localizes to smaller lipid droplets. LSD-1 exclusively locates on lipid droplets while LSD-2 presents both in the cytoplasm and on lipid droplets. However, the roles of these genes in development remain unclear. This present study, we found that LSD-1 expresses not only in wing pouch but also detection in notum of wing imaginal disc. Specific knockdown of Lsd-1 by pannir-GAL4 driver in wing disc leads to split thorax phenotype, suggesting that an essential role of Lsd-1 in development of Drosophila thorax. This role of Lsd-1 is dependent on the activity of the Drosophila c-Jun N-terminal kinase, as overexpression of basket suppresses split thorax induced by Lsd-1 knockdown. Besides these events, depletion of Lsd-1 causes an enhancement apoptosis cell death in wing notum. Moreover, we also demonstrated that knockdown of Lsd-2 led to dramatic cell death and developmental defects in the wing blade. Also, Lsd-2 knockdown-induced apoptosis cell death was shown to be mediated by dFoxO. Taken together, these data indicate Lsd-1 and Lsd-2 have main role in Drosophila development. | |||||||||
| 言語 | en | |||||||||
| 日付 | ||||||||||
| 日付 | 2019-09-25 | |||||||||
| 日付タイプ | Issued | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第933号 | |||||||||
| 学位名 | ||||||||||
| 言語 | ja | |||||||||
| 学位名 | 博士（学術） | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2019-09-25 | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 14303 | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関名 | 京都工芸繊維大学 | |||||||||
