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Study on bacteriophages for suppressing bacteria growth
http://hdl.handle.net/10212/2559
http://hdl.handle.net/10212/25592493157c-40fb-41c3-856f-61e37bc18091
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内容の要約 (171.3 KB)
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内容・審査結果の要旨 (185.5 KB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2022-04-21 | |||||||||
| タイトル | ||||||||||
| タイトル | Study on bacteriophages for suppressing bacteria growth | |||||||||
| 言語 | en | |||||||||
| その他のタイトル | ||||||||||
| その他のタイトル | バクテリアの増殖抑制のためのバクテリオファージの研究 | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
ファン カン ニュイ フン
× ファン カン ニュイ フン
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Escherichia phage | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Taxonomy | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Genome annotation | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Escherichia coli O157:H7 | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Salmonella enterica | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Bacteriolysis | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Lipopolysaccharide | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Outer membrane protein | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Receptor | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Escherichia coli and Salmonella enterica are Gram-negative bacteria. Some strains of both bacteria can cause severe diseases for animals including humans. Ralstonia solanacearum is a soil-borne gram-negative phytopathogenic bacterium causing bacterial wilt disease in many important crops such as rice and tomato. The abuse of antibiotics for treating those pathogens leads to economical effect and increase antibiotic resistance. Bacteriophages (or phages) are viruses which infect and kill specific kind of bacteria with high host specificity. Phage therapy, to cure bacteria infectious diseases using phages, is gaining attention as one of the alternative treatments for bacterial infections. In chapter 1, I isolated 4 kinds of bacteriophages, one is Ralstonia solanacearum phage φRS138 and the three are E. coli phages KIT01, KIT02, and KIT03. The morphological observation of these phages by TEM indicated that KIT01 and KIT03 belong to the family Myoviridae while KIT02 and φRS138 belong to the family Siphoviridae. Genome analysis showed φRS138 is the characteristic of temperate phage with high identity to plasmids of some R. solanacearum. In chapter 2, I analyzed the host range of Escherichia coli phages KIT01, KIT02, and KIT03. These are very unique since they lyse both Salmonella and E. coli. I also analyzed the genome sequence of KIT03 and performed genome annotation and alignment. In chapter 3, I analyzed the phage growth parameters of KIT01, KIT02, and KIT03. The in vitro application of these phages were evaluated to suppress the growth of E. coli and Salmonella. KIT01, KIT02 and KIT03 can cause the bacteriolysis in single apply and synergic effect in cocktail against some strains of E. coli and Salmonella. I further revealed that KIT01 and KIT03 recognizes and adsorbs lipopolysaccharide and outer membrane protein A of bacteria. KIT02 recognizes and utilize BtuB as s receptor with the support of lipopolysaccharide. In conclusion, KIT01, KIT02, KIT03, and these mixtures can be used for controlling both E. coli and Salmonella. | |||||||||
| 言語 | en | |||||||||
| 日付 | ||||||||||
| 日付 | 2020-03-25 | |||||||||
| 日付タイプ | Issued | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第949号 | |||||||||
| 学位名 | ||||||||||
| 言語 | ja | |||||||||
| 学位名 | 博士（学術） | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2020-03-25 | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 14303 | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関名 | 京都工芸繊維大学 | |||||||||
