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Studies on screening for microbes producing vinyl compounds and its application to development of biobased polymers
http://hdl.handle.net/10212/2607
http://hdl.handle.net/10212/26070475dc8e-f33f-46e1-95a3-feeb74d57151
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全文 (13.9 MB)
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内容・審査結果の要旨 (151.1 KB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2024-01-18 | |||||||||
| タイトル | ||||||||||
| タイトル | Studies on screening for microbes producing vinyl compounds and its application to development of biobased polymers | |||||||||
| 言語 | en | |||||||||
| その他のタイトル | ||||||||||
| その他のタイトル | ビニル化合物生産微生物の探索とバイオベースポリマー開発への利用に関する研究 | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
佐野, 芽生
× 佐野, 芽生
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Microbiology | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Alkylitaconic acids | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Itaconic acid derivatives | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | α-Methylene-γ-butyrolactones | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Acrylic acid | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Mizoroki?Heck coupling reaction | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | TLC quantification | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | High-throughput screening | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Itaconic acid | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Aspergillus terreus | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Iodine test | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Thiol-ene reaction | |||||||||
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| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | DISCOVER | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | 9-Hydroxyhexylitaconic acid | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | 10-Hydroxyhexylitaconic acid; Radical polymerization; Renewable polymer | |||||||||
| 主題 | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | Biobased polymer | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | The aim of this study is development of screening method for microbes producing vinyl compounds from environmental samples and synthesis of new biobased polymers using the vinyl compounds as monomers. Thus, the following studies were carried out.In Chapter 1, structure, function, and biosynthesis of itaconic acid (IA) derivatives with the vinylidene group were summarized and their perspectives for industrial use were discussed. In Chapter 2, a selective labeling method based on Mizoroki-Heck reaction for vinyl compounds and a simple TLC?densitometric method for the quantification of the vinyl compounds were developed.In Chapter 3, the selective labeling reaction developed in Chapter 2 was confirmed by an iodine test and the screening of IA-producing fungi from soils was performed using the developed method. Using this method, 37 strains were selected as IA-producing fungi from 240 strains isolated from soils. In Chapter 4, a direct screening method based on Thiol-ene reaction for microbes producing vinyl compounds from soil samples was developed. Soil samples were spread on an agar medium supplemented with TG and VA-044. Screening of soil samples using the screening method led to isolation of IA-producing fungi from a soil. This method was named DISCOVER (direct screening method based on coupling reactions for vinyl compound producers).In Chapter 5, 37 soil microbes isolated in Chapter 3 were further characterized. One out of the 37 strains was identified as A. niger S17-5. Identification of the IA derivatives produced by S17-5 revealed that they were identical to 9-HHIA and 10-HHIA. The antibacterial, anti-inflammatory, and cytotoxic properties of two IA derivatives were evaluated.In Chapter 6, IA and 10-HHIA were copolymerized at different ratios through aqueous free radical polymerization with KPS as an initiator, resulting in poly(IA-co-10-HHIA)s with different monomer compositions. This is the first time that biobased polyvinyls were synthesized using naturally occurring IA derivatives.In chapter 7, general review was described.In conclusion, developed screening methods permit selective isolation of microbes producing vinyl compounds from environment. And the vinyl compounds produced by microbes enable the development of bio-based polymers. | |||||||||
| 言語 | en | |||||||||
| 日付 | ||||||||||
| 日付 | 2020-09-24 | |||||||||
| 日付タイプ | Issued | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第979号 | |||||||||
| 学位名 | ||||||||||
| 言語 | ja | |||||||||
| 学位名 | 博士（工学） | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2020-09-24 | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 14303 | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関名 | 京都工芸繊維大学 | |||||||||
